Organic Acids Mixture

In order to improve the performance of laboratories in organic acid measurements and harmonization of laboratory results, it is essential that control materials are available.

Following advice of the ERNDIM Advisory Board a mixture of organic acids was composed having - after reconstitution with 5 mL water – the following concentrations:

Mixture A µMol/L Mixture B µMol/L
D,L-lactate 2000 glycolate 500
D,L-3-hydroxybutyrate 2000 3-hydroxyisovalerate 500
methylmalonate 2000 ethylmalonate 500
fumarate 500 glutarate 500
adipate 500 L-2-hydroxyglutarate 500
suberate 500 sebacate 500
malonate 500 D,L-pyroglutamate 2000
3-methylglutarate 500 (+/-)-mevalonolactone 500
3-methylglutaconate 500 orotate 500
2-ketoglutarate 500 butyrylglycine 100
isovalerylglycine 500 3-methylcrotonylglycine 100
D,L-2-methylbutyrylglycine 100 hexanoylglycine 100
tiglylglycine 100 N-acetyl-L-aspartate 500
suberylglycine 100 D-(+)-malate 500
propionylglycine 100 D,L-4-hydroxyphenyllactate 500
3-hydroxyglutarate 500 succinylacetone 200
4-hydroxybutyrate 400 thymine 100

The 2 sets of mixtures are provided as lyophilized products. After reconstitution, products should be processed as patient samples according to existing procedures for organic acid analysis.