Organic Acids Mixture
In order to improve the performance of laboratories in organic acid measurements and harmonization of laboratory results, it is essential that control materials are available.
Following advice of the ERNDIM Advisory Board a mixture of organic acids was composed having - after reconstitution with 5 mL water – the following concentrations:
Mixture A | µMol/L | Mixture B | µMol/L |
---|---|---|---|
D,L-lactate | 2000 | glycolate | 500 |
D,L-3-hydroxybutyrate | 2000 | 3-hydroxyisovalerate | 500 |
methylmalonate | 2000 | ethylmalonate | 500 |
fumarate | 500 | glutarate | 500 |
adipate | 500 | L-2-hydroxyglutarate | 500 |
suberate | 500 | sebacate | 500 |
malonate | 500 | D,L-pyroglutamate | 2000 |
3-methylglutarate | 500 | (+/-)-mevalonolactone | 500 |
3-methylglutaconate | 500 | orotate | 500 |
2-ketoglutarate | 500 | butyrylglycine | 100 |
isovalerylglycine | 500 | 3-methylcrotonylglycine | 100 |
D,L-2-methylbutyrylglycine | 100 | hexanoylglycine | 100 |
tiglylglycine | 100 | N-acetyl-L-aspartate | 500 |
suberylglycine | 100 | D-(+)-malate | 500 |
propionylglycine | 100 | D,L-4-hydroxyphenyllactate | 500 |
3-hydroxyglutarate | 500 | succinylacetone | 200 |
4-hydroxybutyrate | 400 | thymine | 100 |
The 2 sets of mixtures are provided as lyophilized products. After reconstitution, products should be processed as patient samples according to existing procedures for organic acid analysis.